Summary: Off-target binding and cleavage by Cas9 pose major challenges in its application. How the conformational dynamics of Cas9 govern its nuclease activity under on- and off-target conditions remains largely unknown. Here. using intra-molecular single-molecule fluorescence resonance energy transfer measurements. https://www.roneverhart.com/Bee-International-Candy-Hearts-Tubes-Mike-Ike-Hot-Tamales-and-Warheads-24-Piece-Display/